Flow Cytometry Crossmatch

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Upon completion of the Flow Cytometry Crossmatch (FCXM) module, the reader should understand:

  • The basic procedures and materials required to perform a FCXM of donor cells for the purpose of detecting circulating donor-specific antibody in pre- and post-transplant patients;
  • The different kinds of flow cytometer data used to interpret FCXM;
  • The effects of different therapeutic agents, cell preparation methods and reagents on the FCXM assay;
  • Relative nature of the terms “positive FCXM” and “negative FCXM”; and
  • How to recognize and resolve problems encountered when performing the assay.