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Unfortunately, at this time the feature that links the CE's from ASHI-U to Learning Builder is currently disabled.  You will have to check the box that you don't have an ABHI Account.  You can complete the Modules on ASHI-U but you will have to manually input the CE's into Learning Builder.  We are so sorry for the inconvenience.  We are working diligently to get this issue resolved as soon as possible.

Flow Cytometry Crossmatch

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Objectives:

Upon completion of the Flow Cytometry Crossmatch (FCXM) module, the reader should understand:

  • The basic procedures and materials required to perform a FCXM of donor cells for the purpose of detecting circulating donor-specific antibody in pre- and post-transplant patients;
  • The different kinds of flow cytometer data used to interpret FCXM;
  • The effects of different therapeutic agents, cell preparation methods and reagents on the FCXM assay;
  • Relative nature of the terms “positive FCXM” and “negative FCXM”; and
  • How to recognize and resolve problems encountered when performing the assay.