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Antibody Detection by ELISA

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Objectives:

  • Understand and identify the components of the ELISA antibody identification assay including the target antigens, secondary antibodies, enzyme substrates and their by-products.
  • Identify a positive or negative reaction, understand how this determination was obtained, and how it applies to the clinical assessment of the transplant candidate.
  • Understand and identify the ASHI standards that pertain to the compliance of the assay, the plate reader and automated washer, and the quality control measures incorporated in the test system.