Objectives:

• Identify the organs that are home to immune cells
• Describe the various cells arising from a hematopoietic stem cell and their characteristics 

CEUs Earned: 0.15

Objective:

• List important cells and tissues of the immune system and describe how cell types are distinguished from one another.

CEUs Earned: 0.15

Objective:

• Diagram the structure of an antibody molecule and label the regions
• List the immunoglobulin classes/isotypes and subclasses

CEUs Earned: 0.15

Objectives:

• Define the complement system
• List the three complement pathways

CEUs Earned: 0.15

Objectives:

• Compare and contrast the properties of the HLA class I and class II proteins
• Describe the role that HLA plays in immune response to pathogens

CEUs Earned: 0.15

Objectives:

• Describe the general properties of cytokines
• Appreciate the role of cytokines in the development of immune cells and in innate and adaptive immune responses

CEUs Earned: 0.15

PURPOSE OF THE MANUAL

The purpose of this manual is to provide the reader with the basic background needed to understand the HLA system and the molecular biology methods used to identify HLA "types".

CEUs Earned: 0.00

Objectives:

• Identify the different fields in DNA nomenclature
• Describe the relationship between HLA serology and DNA nomenclature

CEUs Earned: 0.15

Historical Highlights in Histocompatibility and Transplantation - Julius M. Cruse Jr., M.D., Ph.D.

CEUs Earned: 0.15

Objectives

Students should be able to:

•           Describe the major developments in the field of transplantation

CEUs Earned: 0.15

Objectives:

Students should be able to:

• Describe the purpose of the International Histocompatibility Workshops
• Describe major advances made within the Workshops

CEUs Earned: 0.15

Objectives:

• Define terms for various donor sources and outcomes of transplantation
• Describe the targets of the immune response to transplanted tissue/cells/organs
   

CEUs Earned: 0.15

Objectives:

• Understand sample selection and DNA extraction procedures
• Maximize quantity and quality of total DNA obtained in the shortest timeframe

CEUs Earned: 0.15

Objective:

• Understand how to quantify extracted genomic DNA (dsDNA) and determine its purity using either UV absorbance spectroscopy or intercalating fluorochromes assays.

CEUs Earned: 0.15

Objectives:

• Understand how to prepare a gel.
• Understand how to perform gel electrophoresis.

CEUs Earned: 0.15

Objectives:

• Describe what primers are and how they are used for HLA typing
• Understand assay conditions that influence the specificity and sensitivity of primer-based assays

CEUs Earned: 0.15

Objective:

• Understand and identify the ASHI standards that pertain to the maintenance and monitoring of the PCR environment.

CEUs Earned: 0.15

Objectives:

• Describe what DNA probes are and how they are used for HLA typing
• Understand assay conditions that influence the specificity and sensitivity of probe hybridization

CEUs Earned: 0.15

Objectives:

• Understand how the SSP assay for HLA typing works
• Understand and identify the ASHI standards that pertain to DNA amplification based typing.

CEUs Earned: 0.15

Objectives:

• Understand the basic technique used to perform rSSO testing
• Understand how to interpret test results and provide the final answer: the patient’s HLA typing result

CEUs Earned: 0.15

Objectives:

• Understand the basic protocol for reverse sequence specific oligonucleotide (rSSO) probing for HLA typing.
• Understand the science behind the protocols.

CEUs Earned: 0.15

Objectives:

• Explain how DNA sequencing with chain terminators works
• List the various strategies for sequencing HLA and give their pros and cons

CEUs Earned: 0.15

Objectives:

• Describe newer methods of genomic DNA sequencing
• Compare Next Generation Sequencing (NGS) to Sanger sequencing

CEUs Earned: 0.15

Objectives:

• Describe how novel HLA alleles are first observed and characterized in the laboratory
• Describe how novel sequences are assigned an allele designation
   

CEUs Earned: 0.15

Objectives:

• To identify aspects of histocompatibility work where application of informatics approaches can be helpful

CEUs Earned: 0.15

Objectives

• Understand the basics of real-time PCR (polymerase chain reaction) assays for HLA typing

CEUs Earned: 0.15

Objective:

• Understand various cell isolation techniques & appropriate uses for specific assays

CEUs Earned: 0.15

Objectives:

• Describe the purpose and applications of cell cryopreservation in histocompatibility testing.
• Identify factors that affect the viability of thawed cells.

CEUs Earned: 0.15

Objective:

•  To understand the clinical rationale for using various types of serum treatments for HLA antibody screening and/or crossmatching by the CDC method.

CEUs Earned: 0.15

Objectives:

• Recognize when interfering substances are present and affecting HLA antibody testing 

CEUs Earned: 0.15

Objectives:

• To describe the history and development of the microlymphocytotoxicity assay and its use in serologic HLA typing.

CEUs Earned: 0.15

Objective:

• Describe the microscopes used to read and grade serologic HLA test reactions.

CEUs Earned: 0.15

Objective:

• Understand basic procedures & materials required to perform a FCXM of donor cells 

CEUs Earned: 0.15

Objective:

• Understand and identify the components of the ELISA antibody identification assay including the target antigens, secondary antibodies, enzyme substrates and their by-products.

CEUs Earned: 0.15

Objective:

• To describe the regulations that apply to histocompatibility and immunogenetics laboratories in the United States. 

CEUs Earned: 0.15

Objective:

• Describe the purpose of a quality management system

CEUs Earned: 0.15

Objectives:

• Describe measurements used in the laboratory and relationships among measurements
• Describe the math used in the laboratory to prepare reagents or to characterize results

CEUs Earned: 0.15

Objective:

• Understand and identify ASHI standards that pertain to Confidentiality, Test request, Test report, and Test records.

CEUs Earned: 0.15

Objectives

To describe the requirements for equipment maintenance and calibration as an important part of the laboratory’s quality assurance (QA) program based on ASHI Standards, as well as good laboratory practice.

CEUs Earned: 0.15

Objectives:
• Explain the role of killer cell immunoglobulin-like receptors in detecting the
loss of HLA class I on the cell surface
• Utilize KIR nomenclature

CEUs Earned: 0.15

Objectives:

• Obtain an understanding of the clinical problems associated with antibodies to neutrophils
• Become familiar with the human neutrophil antigen (HNA) system

CEUs Earned: 0.15

Objectives:

• Describe the biology and genetics behind the ABO blood group
• Determine the inheritance of ABO in a family
• Describe the tests that determine ABO

CEUs Earned: 0.15

Objectives:

• Understand what “chimerism” refers to in the hematopoietic stem cell transplant (HSCT) setting

• Understand how chimeric mixtures are evaluated using CE data

CEUs Earned: 0.15

Objectives:

• Describe the purpose and process of hematopoietic stem cell transplantation
• List the various sources of hematopoietic stem cells

CEUs Earned: 0.15

Objectives:

• Explain platelet refractoriness
• Describe clinical and laboratory features
• Describe transfusion support for platelet refractoriness

CEUs Earned: 0.15

Objectives:

• Understand what is a virtual crossmatch, the need for it and how it is currently being used in transplantation

CEUs Earned: 0.15

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary case from Dr. Nicholas Brown, University of Chicago

Exemplary cases from Dr. Rob Liwski, Nova Scotia Health Authority HLA Laboratory

Exemplary cases from Dr. Rob Liwski, Nova Scotia Health Authority HLA Laboratory

An Introduction to ASHI - Rebecca L. Upchurch, MLT (ASCP), CHS

CEUs Earned: 0.15

The Evolution of Risk Assessment in Solid Organ Transplantation - Kelley M.K. Hitchman, MS, PhD, D(ABHI)

CEUs Earned: 0.15

Application of the Virtual Crossmatch in Organ Allocation - Debra Kukuruga, MT(ASCP), PhD, D(ABHI)

CEUs Earned: 0.15

News and Views of HLA Epitope Mapping - "Are we ready for the new frontier?" - Debra Kukuruga, MT(ASCP), PhD, D(ABHI)

CEUs Earned: 0.15

HLA Lab Assays: The Past, Present, and Future Directions

CEUs Earned: 0.15

Antibody Mediated Graft Rejection in Solid Organ Transplantation - Jinguo Wang, PhD, D(ABHI)

CEUs Earned: 0.15

Anti-AT1R Antibodies in Abdominal Solid Organ Transplantation: When Should We Test? - Kelley M.K. Hitchman, MS, PhD, D(ABHI)

CEUs Earned: 0.15

Haploidentical Stem Cell Transplantation - Jinguo Wang, PhD, D(ABHI)

CEUs Earned: 0.15

CHT/CHS Certification Exam Review - Brittany Delgadillo, BS, CHT(ABHI)

CEUs Earned: 0.15

Basics of Transplant Immunology - Luis Hidalgo, PhD, D(ABHI)

CEUs Earned: 0.15

How Does Patient Treatment Impact Antibody Test Results Pre-Transplant? - Matthew Cusick, PhD, D(ABHI)

CEUs Earned: 0.15

Immunologic Reasoning Behind HLA Antibody Testing Methods - Luis Hidalgo, PhD, D(ABHI)

CEUs Earned: 0.15

Interpretation of MFI in Antibody Testing - Matthew Cusick, PhD, D(ABHI)

CEUs Earned: 0.15

Typing HLA by NGS: How Immunogenetics Labs Target a Complicated Genetic System - Nicholas K. Brown, PhD, D(ABHI)

CEUs Earned: 0.15

NGS HLA Typing for Solid Organ Transplantation - Gerald P. Morris, MD, PhD

CEUs Earned: 0.15

CHT/CHS Certification Exam Q&A

CEUs Earned: 0.15

Complement Modifications to Solid Phase Antibody Testing - Nicole Valenzuela, PhD

CEUs Earned: 0.15

Desensitization Protocols and Test Interference - Karl Schillinger, MS, CHS

CEUs Earned: 0.15

HLA Antibodies in Hematopoietic Stem Cell Transplantation - Jennifer Schiller, PhD, D(ABHI)

CEUs Earned: 0.15

HLA Antibodies In Solid Organ Transplantations - Amy Hahn, PhD, D(ABHI)

CEUs Earned: 0.15

HLA Typing by NGS - Jennifer Schiller, PhD, D(ABHI)

CEUs Earned: 0.15

HLA Diseases and Drugs - Amy Hahn, PhD, D(ABHI)

CEUs Earned: 0.15

Non-HLA Antibodies: Test Interference Targets and Detection for Solid Organ Transplant - Nicole Valenzuela, PhD

CEUs Earned: 0.15

Virtual Cross Matches Living in the World of KAS - Karl Schillinger, MS, CHS

CEUs Earned: 0.15

Overview of Deceased Donor Organ Donation - Sam Ho, PhD, D(ABHI)

CEUs Earned: 0.15

Testing for Non-HLA Antibodies in the Lab - Michele Prod, MT(ASCP), CHS(ABHI)

CEUs Earned: 0.15

The New OPTN Kidney Allocation System - Sam Ho, PhD, D(ABHI)

CEUs Earned: 0.15

NK Cell Allorecognition in Transplantation - Zahra Kashi, PhD, HCLD

CEUs Earned: 0.15

The Immunology of Transplant Rejection - Peter Jindra, PhD, D(ABHI)

CEUs Earned: 0.15

HLA Beyond Transplantation - Michele Prod, MT(ASCP), CHS(ABHI)

CEUs Earned: 0.15

Debate: Relevance of Complement Dependent Cytotoxicity (CDC) Methods

CEUs Earned: 0.15

Role of HLA in Pathogenesis and Diagnosis of Celiac Disease - Manish Gandhi, MD

CEUs Earned: 0.15

What to Expect When You are Expecting (An ASHI Inspection) - John Schmitz, PhD, D(ABMLI, ABHI)

CEUs Earned: 0.15

Hematopoietic Stem Cell Transplantation From Indications to Donor Selection - Carolyn Hurley, PhD, D(ABHI)

CEUs Earned: 0.15

Applying HLA Informatics Tools to an Unrelated Donor Search - Carolyn Hurley, PhD, D(ABHI)

CEUs Earned: 0.15

Virtual Crossmatch: Where Do We Stand? - John Schmitz, PhD, D(ABMLI, ABHI)

CEUs Earned: 0.15

UPMC Tissue Typing Laboratory The Case of The Mysterious HLA Typing - Georgianna Kross

CEUs Earned: 0.15

HLA Typing Anti-HLA Antibodies and Crossmatch Cases- Manish J. Gandhi, MD

CEUs Earned: 0.15

Flow Crossmatch Variables – What’s in Our Control? - Anne Halpin, MSc, CHS

CEUs Earned: 0.15

Platelet Allo-Antigens: What Are They and What Do They Have to Do With the HLA Laboratory? - Anne Halpin, MSc, CHS

CEUs Earned: 0.15

ASHI Access is your online portal to educational information from a variety of sessions from the ASHI/Banff Joint Scientific Meeting. This portal will allow you to view session PowerPoint presentation videos with the speaker over voice.

CEUs Earned: 0.00

ASHI Access is your online portal to educational information from a variety of sessions from the ASHI/Banff Joint Scientific Meeting. This portal will allow you to view session PowerPoint presentation videos with the speaker over voice.

CEUs Earned: 0.00

Members must log in to view the newest edition of the ASHI Quarterly- https://www.ashi-hla.org/page/ASHI_Quarterly

CEUs Earned: 0.45

ASHI Journal Club #1 - Donor-Derived Cell-Free DNA Predicts Allograft Failure and Mortality after Lung Transplantation

CEUs Earned: 0.15

ASHI Journal Club #2 - Overview of Directors' Training Review and Credentialing (DTRC) Process

CEUs Earned: 0.15

ASHI Journal Club #3 - CXCR5+PD1+ICOS+ Circulating T Follicular Helpers Are Associated With de novo Donor-Specific Antibodies After Renal Transplantation

CEUs Earned: 0.15

ASHI Journal Club #4 - DIT Career Development

Anat Tambur, Northwestern University.

Anna Greenshields, Halifax HLA Laboratory, Nova Scotia Health Authority.

Carol Pancoska - Facilitator

August 12, 2020

CEUs Earned: 0.15

The Professional Standards Division is comprised of the Quality Assurance and Standards Committee, Accreditation Review Board, Proficiency Testing Committee, and the Directors' Training Review and Credentialing Committee.

CEUs Earned: 0.23

Zahra Kashi, Kashi Clinical Laboratory/ Julie Houp, University of Alabama at Burmingham/ Michael Gautreaux, Wake Forest School of Medicine/ Anne Halpin, University of Alberta Hospital.

ASHI Webinar, 2020

CEUs Earned: 0.23

Anne Halpin (Moderator), University of Alberta Hospital/ Medhat Askar, Baylor University Medical Center/ John Schmitz, University of North Carolina School of Medicine/ Zahra Kashi, Kashi Clinical Laboratory

ASHI Webinar, 2020

CEUs Earned: 0.15

Jason Dehn, NMDP / Carolyn Hurley, Georgetown University

ASHI Webinar, 2020

CEUs Earned: 0.23

William Hilderbrand, University Of Oklahoma Health Science Center/ Martin Malers, Be The Match/National Marrow Donor Program/ Annette Jackson, Duke University School of Medicine/ Zachary Antovich, Thermo Fisher... (more)

CEUs Earned: 0.15

Bjoern Peters
La Jolla Institute for Allergy and Immunology

CEUs Earned: 0.15

Todd M. Allen, Ph.D.
Professor of Medicine, Ragon Institute of MGH, MIT, and Harvard

CEUs Earned: 0.15

Hannah Carter, PhD
University of California, San Diego
Department of Medicine

CEUs Earned: 0.15

Elaine F. Reed, Ph.D.
Elaine and Daljit Sarkaria Endowed Chair in Diagnostic Medicine
Professor of Pathology and Laboratory Medicine
Director, ULCA Immunogenetics Center
David Geffen School of Medicine at UCLA

CEUs Earned: 0.15

Melanie Harriff, PhD
Research Assistant Professor, Oregon Health & Science University
Research Microbiologist, Portland VA Health Care System 

CEUs Earned: 0.15